Idizayini yomuthi wokugoma we-Peptide osuselwa ku-Multiepitope ngokumelene ne-E Protein ye-Human COVID-19:

Indlela Ye-Immunoinformatics

Isendlalela.

Isifo esisha esingapheli sesibhebhetheke edolobheni lase-Wuhan, eShayina, ngoZibandlela/Disemba 2019.

Emasontweni am’balwa, inhlangano yezempilo emhlabeni wonke (WHO) imemezele isifo esisha somkhuhlane wokhuvethe sango-2019 sabizwa nge-COVID-19.

Sekuphela uMasingana/Januwari 2020, i-WHO imemezele ukuqubuka “kwesimo sezempilo esiphuthumayo emhlabeni wonke” ngenxa yokubhebhetheka ngokushesha kwalesi sifo emhlabeni wonke.

Okwamanje, awukho umgomo ophasiselwe lesi sifo esiqubukayo; ngalokho, inhloso yalolu cwaningo ukwakha umgomo onama-epitope amaningi ozolwa ne-COVID-19 kusetshenziswe i-immunoinformatics.

Indlela yokwenza.

Kusetshenziswe amasu amaningana asiza inhlanganisela yendlela ye-immunoinformatics kanye nendlela yokuqhathanisa ye-genomic ukuze kutholwe ama-peptide angaba khona wokuklama umuthi wokugomela we-peptide osuselwa ku-T-cell kusetshenziswa iphrotheni yemvilophu ka-2019-nCoV njengokuhlosiwe.

Imiphumela.

Ukuguqulwa okubanzi, ukufakwa, nokususwa kutholwe ngokulandelana okuqhathaniswayo ngokohlobo lwe-COVID-19.

Ngaphezu kwalokho, ama-peptide ayishumi abophezela ekilasini le-MHC I ne-MHC yekilasi II atholwe njengabafundi abathembisayo bomklamo wokugoma onenani elanele labantu emhlabeni wonke elingu-88.5% no-99.99% ngokulandelana.

Isiphetho.

Umuthi wokugomela i-peptide osuselwa ku-T-cell wenzelwe i-COVID-19 kusetshenziswa iphrotheni yemvilophu yokuhloswe yi-immunogenic.

Noma kunjalo, umuthi wokugoma ohlongozwayo udinga ukuqinisekiswa ngokushesha emtholampilo ukuze kuqinisekiswe ukuphepha kwawo kanye nephrofayili ye-immunogenic ukusiza ukumisa le mpathamhlaba ngaphambi kokuthi kuholele ekuqubukeni okubhubhisayo komhlaba.

Abacwaningi baseSudan bakha umuthi wokugomela i-COVID-19 ongaba khona

Ngo-2020, ngaphambi kokuthi imigomo yokugomela i-COVID-19 itholakale kalula, abacwaningi baseSudan bahlongoze umuthi wokugoma ozovuselela ukuzivikela komzimba ngokumelene nephrotheni yemvilophu yegciwane le-SARS-CoV-2.

Ngaleso sikhathi, abacwaningi abaningi bebefuna imigomo ephephile nesebenzayo yokugomela i-COVID-19, eyayisanda kuqala ukusabalala ngokushesha emhlabeni wonke jikelele.

Imithi yokugoma esekelwe ku-peptide iyindlela ephephile futhi eshibhile uma kuqhathaniswa nezinye izinhlobo zemithi yokugoma.

Ama-peptide ayizintambo ezimfishane zama-amino acid futhi lawa ma-amino acid ayizakhi zamaprotheni, aqukethe ama-peptide amaningi.

I-epitope iyi-peptide eqondiwe engabonwa amasosha omzimba.

Imithi yokugoma esekelwe ku-peptide isebenzisa ama-epitopi asuka engxenyeni yegciwane ebangela ukusabela komzimba emzimbeni, kulokhu iphrotheni yemvilophu, noma iphrotheni engu-E.

Iphrotheni engu-E iyingxenye yesikhuma sephrotheni (“ikhava”) esizungeze igciwane.

Le phrotheni idlala indima ebalulekile endleleni igciwane elithelela ngayo izakhi zomzimba nokuthi lizidala kanjani izifo.

Abacwaningi kulolu cwaningo bahlose ukuklama umgomo osuselwa ku-peptide ngokuqhathanisa ukulandelana kofuzo kwama-peptide okwabiwe phakathi kwezinhlobo ezahlukahlukene ze-coronavirus, kusetshenziswa amaprotheni emvilophu (E) njengokuhlosiwe.

Okokuqala baseshe isizindalwazi ukuze bathole ukulandelana kwezakhi zofuzo okungase kuqondiswe kuma-peptide.

Okulandelayo, basebenzise amamodeli ekhompiyutha kanye nolwazi lwabo ngokuthi imithi yokugoma ivuselela kanjani ukusabela komzimba ukuze babone ukulandelana kofuzo okungasetshenziswa ukuklama umgomo.

Abacwaningi bakwazile ukukhomba inqwaba yama-peptide okwabelwanwa ngawo phakathi kwezinhlobo ezahlukene zokhuvethe ezinamandla okusetshenziswa njengemgomo, kodwa bazinza ku-E protein njengekhandidethi lokugoma.

Bathi baklame ngempumelelo umgomo we-COVID-19 osuselwa kumaprotheni besebenzisa ukulandelana kwaleli phrotheni lemvilophi.

Ngaleso sikhathi, abacwaningi bathi babengabokuqala ukuhlonza izingxenye zemvilophu (E) protein (peptides) njengabazogomela i-COVID-19.

Abakutholile kugqamise amandla emigomo esekelwe kumaprotheni, ebiphephile, entsha, engabizi futhi eyindlela esebenzayo yokwenza umgomo we-COVID-19.

Nokho-ke, ikhandidethi labo lokugoma belizodinga ukuthi liphinde lihlolwe endaweni yomtholampilo ukuze kuqinisekiswe ukuthi uphephile futhi yasebenza.

Ngokusho kwethimba labacwaningi baseSudan abenza lolu cwaningo, imithi yokugoma amaprotheni ibiza kancane ukuyithuthukisa, ngakho-ke inamandla amakhulu okuthuthukiswa futhi isatshalaliswe emazweni asathuthuka.